Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. International Journal of Biological Macromolecules, 42, 133-137. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. ; Fran, H.P. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. 2019;26(1):25261. @ Nilimesh See attached. Molecular weight determination of polysaccharides. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Huggins equation [41] gives the relation to the relative viscosity increase (i). Chem. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Banga AK. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Bethesda, MD 20894, Web Policies Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Journal Argentine Chemical Society 100 (2013) 25-34. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. 2008;28(5):64557. Advances in chemistry series Washington, DC: American Chemical Society., pp. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. Epub 2017 Jun 1. Carbohydrate Polymers, 38, 267-271. TEWL measurements were taken 30min after microporation of the human and pig skin. Determination of intrinsic viscosities of macromolecules and nanoparticles. 1994, 95-0355. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). National Library of Medicine This result could reflect the presence of some aggregated species within the lysozyme sample solution. Journal of American Chemical Society, 76 (20), 5041-5052. CAS Citation-(RIS dextran To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. (1990). Theory, Estimation, Experiment, and Data. dextran hydrodynamic radius principles of dynamic light scattering ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Catiker, E.; Guner, A. ; Weisz, J. Structure Properties of Dextran. 2015;23(3):2239. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Carbohydrate Polymers 99, 199-207. Macromolecules. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Recent Pat. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. (2004). Not for use in diagnostic procedures. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Rotureau, E.; Dellacherie, E.; Durand, A. dextran government site. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. osmotic pressure or light scattering). ; Garca de , J. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Prausnitz MR, Langer R. Transdermal drug delivery. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Walkenhorst, S.; Olivier, R. (2003). where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. HHS Vulnerability Disclosure, Help Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Journal of Polymer Science, 17, 527-546. Progress in Polymer Science, 30, 38-70. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Clipboard, Search History, and several other advanced features are temporarily unavailable. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. All branched polysaccharide types could be described approximately by the hyperbranching theory. Dextran Fractions. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. We observe that this maximum radius is below Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Soc., 21, 1, 78-86. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Dextran 2016;47:15964. Transdermal delivery of FITC-Dextrans with different molecular Modelling the endothelial blood-CNS barriers: a method for the The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Increased TEWL values are evidence of micropores formation. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Transdermal drug delivery: overcoming the skins barrier function. dextran Materiaux 1-5. Critical parameters for gel permeation The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Careers. NCI CPTC Antibody Characterization Program. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Masuelli, Martin Alberto (2013). Pharm Res. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Macromolecules, 33, 5730-5739. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. (2008). Small Intestinal Permeability and Gut-Transit Time Determined Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence There is a deviation from linearity after 580kDa [69, 70]. Radiofrequency ablation of malignant liver tumors. For data requests, please contact the authors. Macromolecules, 31, 7712-7716. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. ; Yevlampieva N.P. Dextran. Webwhittier union high school district superintendent. European Polymer Journal, 42, 1086-1092. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. This conjecture found to be correct. Environ Toxicol Pharmacol. Ubbelohde, Leo. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Formul. IV. If there are particles in the sample, the incident laser light gets scattered in all directions. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Probiotics Antimicrob Proteins. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. All authors read and approved the final manuscript. ; Veldhuis, T.F.J. Polymer molecular weight distribution. Advances in Physical Chemistry, Vol. Pavlov, G.M. (1987). (1954). The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). 8580 Macromolecules 34, Notes - NIST The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Table 2 compares the properties of nanoparticles coated with one or other polymer. Treatment of acne scars by fractional bipolar radiofrequency energy. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 4D imaging reveals mechanisms of clay-carbon protection and Microneedles and their applications. American Journal of Food Science and Technology 2013, 1(3), 60-66. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. The https:// ensures that you are connecting to the Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Biomaterials, 22, 1891-1898. Ioan, C.E. PubMedGoogle Scholar. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Ther. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Fluorescent dextran Please enable it to take advantage of the complete set of features! Sci. Harding, S. E., & Simpkin, N. J. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). The micropore formed at the SC through to the epidermis, enabling drug delivery. Epub 2022 Feb 2. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Study of temperature influence. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). 2004;25(9):155967. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. The Viscosity of Dilute Solutions of Long-Chain Molecules. Carbohydrate Polymers 72, 356-360. Dextran Polymers | Free Full-Text | Confinement-Induced Fractionation and Bohdanecky, Miloslav (1996). Fixable: Fixable via Lysine. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. In fact, short chains do contain minor branching points. Epub 2012 Oct 18. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Terms and Conditions, The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. This site needs JavaScript to work properly. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. ; Rjumtsev, E.I. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. 2019;9(3-S):9726. dextran Accessibility US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES (2003). Beer, M.U. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Cite this article. (2000). In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). He, L.; Niemeyer, B. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Drug Deliv Syst. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Smith, David R.; Raymonda, John W. (1972). US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. (1937). Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Data File Dextran. Unauthorized use of these marks is strictly prohibited. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation.
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